HATCHERY VACCINATION 2.3 Marek’s Vaccine Storage Marek’s Disease vaccine is a very unique vaccine in that they are live viruses that are cell associated and kept frozen in liquid nitrogen. The vaccines must be carefully thawed and mixed prior to administration in any form. The nitrogen tanks must also be properly maintained to ensure the vaccines stay at a constant temperature. 2.4 Nitrogen Storage Tank Maintenance » Always wear safety glasses and insulated gloves while handling vaccine from liquid nitrogen containers and measuring the level of nitrogen in the tank.
COBB VACCINATION MANAGEMENT GUIDE » Avoid moving the storage tank abruptly and avoid falls or bumps, which might break the internal walls and/or neck tube, resulting in loss of tank vacuum and/or total loss of liquid nitrogen. » The tank must be stored in a cool place, away from direct sunlight and any other heat sources. » The tank lid must be placed correctly (see photo on right). » After using all the vaccine ampules, do not allow all the nitrogen to evaporate from the tank. Putting liquid nitrogen in an empty tank can cause damage and tank failure. X » Handle the tank with both hands, keeping it in an upright position. Do not lift the tank with one hand.
» Vaccine ampules must always be submersed in the liquid nitrogen. » The level of liquid nitrogen must never be under 30 cm (11.8 in), as measured with a suitable ruler. The nitrogen level must be checked daily. » Use Personal Protective Equipment (PPE) when measuring the level of nitrogen. 8