., . ., - ,.. Downloaded from https://www.everyspec.com . . .MIL-STD-81OC .- (3) Dip the cotton strips into the above solution. After dipping, remove excess liquid from the strips and hang them to dry before placing them in chamber and inoculating th+. the the ) (4) Within the chamber, place the strips vertically in close proximity to bracketing the test item so that the test strips and test items experience and the same test environment. The length of the strips shall be at least the height of the test item. 11.3.3 ms.ad.U. Test Performance 11.3 .3.1 Preparation for incubation a.
Assure that the condition of the test items subjected to testing is similar to that as delivered by the manufacturer or customer for use, or as otherwise specified. Any oleaning of the test item shall be accomplished at least 72 hours prior t.othe beginning of the fungus test. b. Install the test item in the chamber or cabinet on suitable fixtures or suspended from hangers. c. Hold the test item in the operating ohamber for at least 4 houra immediately prior to inoculation. d.
Inoculate the test item and cotton fabrio chamber control items (11.3.2.l.3b(l) ~th the mixed fung~ apcre suspension (11.3.2.1.2) by spraying it on the control and on and into the test item(s) (if not hermetically sealed)1 in the form of a fine mist frcm an atomizer or nebulizer. m: In spraying the test and control items with composite spore suspension care should be taken to cover all external and Internal surfaces which are exposed during use or maintenance. If the surfaces are nonwetting, spray until initiation of droplet coalescence. e. Replace covers of the test items loosely. f. Start incubation immediately following the inoculation. 11.3.3.2 Incubation of the test item. a.
Incubate the test item(S) under a daily cycle of temperate and humidity conditions consisting of 20 hours of a relative humidity of 95 f 5 Percent at an air temperature of 30° ~ 1°C (86° ~ 2° F) followed by a 4-hoQr period in which conditions of 95 ~~ percent reiative humidity at 25° f 1° C (77° ~ 2° F) are maintained for at least 2 hours. Up to a total of 2 hours of the 4-hour period will be used for the transition S) of temperate and relative humidity. Temperature and humidity conditions during the transition periods must be as follows: temperature 24° to 31° C (75° to 880F) and relative humidity above 90 percent. b. Repeat the 24-hour daily cycle for the test dmation. c.
After 7 days, inspect the growth on the control cotton strips to assure that the environmental conditions in the chamber are suitable for growth. lpersonnel with appropriate knowledge of the test item should be available to aid in exposing Ita interior surfaces for inoculation. METHOD 508.2 , 508.2-13