Downloaded from https://www.everyspec.com MIL-STD-810C Magnesium sulfate heptahydrate (MgS04.7H20) . . . . . . . . . . . ..0.7g Ammonium nitrate (NH4N03) . . . . . . . . 1.0 g Sodium chloride (NaCl) . . . . . . . 0.005 g Ferrous sulfate heptahydrate (FeS04.7H20) . . . . . . . . . . . . 0.002 g Ziricsulfate heptahydrate (ZnSOq.7H20) . . . . . . . . . . . . . 0.OO2 g Manganous sulfate monohydrate (MnS04.H20) . . . . . . . . . . . . . .O. Oolg Distilled water . . . . . . . . . . . . . 1000 ml Sterilize the mineral salts solution by autoclaving at 121° C (250”F) for 20 minutes.
Adjust the pH of the solution by the addition of O.01 normal solution of NaOH so that after sterilizationthe pH is between 6.0 and 6.S. Prepare sufficient salts solution for the required tests. 3.1.1.1 ~. Reagent grade chemicals shal1 be used in al1 tests. Unless otherwise specified, it is intended that all reagents shall conform to the specificationof the Committee on Analytical Reagents of the American Chemical Society, where such specificationsare available. 3.1.1.2 Purity of water. Unless otherwise specified, references to water I shal1 be understood to mean disti1led water or water of equal purity. 3.1.2 Preparationof mixed spore suspension. The following test fungi shall be used:
ATCC QM(NLABS) @l.@ No. 1/ No. ~/ - Aspergillus niger 9642 386 Aspergillus flavus 9643 380 Aspergillus versicolor 11730 .432 Penicillium funiculosum 11797 474 Chaetomium globosum 6205 459 ~/ American Type Culture Collection, 12301 Parklawn Drive, Rockville, Maryland 20852 I ~/ Pioneering Research Division, U.S. Army Natick Laboratories,Natick, Massachusetts 01760 METHOD 508.1 508.1-2 !