Downloaded from https://www.everyspec.com :. ~ MIL-STD-81Oc ) n. Repeat this operation for each organism used in the test. o. Perform a viability check for each organiam in accordance with”11.3.Z.1.Sa. P. Blend equal volumes of the resultant spore suspension to obtain the final mixed spore suspension. N5@: The spore suspension may be prepared fresh. If not ~eshly prepared, it should be held at 6° ? 4°C (43° ? 7°F) for not more than 7 days. II.3.2.1.3 Control iteina. TWO types of control procedure of 11.3.3.1.Sa verify the viability of preparation. Using the procedure of II.3.2.1.Sb chamber environment. tests are required. Using the spore suspension and its verify the suitability of the a.
Viability o“fspore euepension. (1) Prior to preparing the composite spore suspension inoculate sterile Ptato dextrose agar plates with ,0.2to 0.3 milliliters of the spore suspension of each of the individual fungal species using separate wtato dextrose ~ar platea for each species. (2) Distribute the inoculum over the entire wirfaoe of the plate. (3) Incubate the Inoculated rmtato dextrose (75° to 88° F) for 7 to 10 days. agar plate at 24° to 31° C (4) After the Incubation period, check the fungal growth. J.@LQ: The abaence of copious growth of any of the test organisms over the entire surface in each container will invalidate the results of any tests using these spores. b.
Test ohamber environment. (1) Inoculate a known susceptible substrate along with the test item to insure that proper conditions are present in the incubation chamber to promote fungal growth. ~: The controlled substrate,shall consist of cotton fabric strips conforming to MIL-T-43566A Tape, Textile, Cotton, General Purpose, Natural or in Colors, Type la, Class 2, bleached, white flat construction. / (2) Prepare the following solution:
(a) 10.O grams glycerol (b) 0.1 potassium dihydrogen orthophosphate (KH2P04) (c) o.lg ammonium nitrate (NH4N03) (d) 0.025g magnesium sulfate (M3S04.7 H2) (e) O.05s yeaat extract (f) Distilled water to a total volume of 100 ml (g) “ HC1 or NaOH to adjust the final solution PH to 5.3. MSTHOD ‘508.2 508.2-12 . f