Downloaded from https://www.everyspec.com MIL-STD-810D 19 July 1983 a. Using aseptic techniques, least the following test fungi: prepare the spore suspension containing at Table 508. 3-II. l.s.atfunai.. ‘Fungi Fungus Sources Identification No. USD~/ ATC&’/ 1 ~ni&2r QM ,386 ATCC 9642 ; QM 380 ATCC 9643 xfmicOlor QM Q32 ATCC 11730 . . .
~ $-lJQIOulosusl QM 474 ATCC 11797 QM 459 ATCC 6205 ~/ US Department of Agriculture (SEA/FR) Northern Regional Research Center ARS Culture Collection 1815 North University Street Peoria, Illinois 60604 (The fungi may be distributed in a lyophilized state or on agar slants.) 2/ American Type Culture Collection 12301 Parklawn Drive Rockville, Maryland 20852 1 - b. Maintain pure cultures of these fungi separately on an appropriate medium such as potato dextrose agar, but culture CheetO ium m globosuston atrips of filter paper overlaid on the surface of mineral salts agar. c. Prepare mineral salts agar by dissolving 15.Og of agar in a liter of tbe mineral salts solution described in II-3.1.1.
NOTE: Do not keep the stock cultures for.more than 4 months at 60 ~ 40c (430 ~ 70F); after that time, prepare subculturesand use them for the new stocks. d. Verify the.purityof fungus cultures before the test. e. Incubate subcultures used for preparing new stock cultures or the spore suspension at 300 + 1.4° C (86° ~ 2.5° F) for 14 to 21 days. f. Prepare a spore suspension of each of the five fungi by pouring into one subculture of each fungus 10 ml of an aqueous solution containing.O .05g Per liter of a nontoxic wetting agent such as sodium dioctyl sulfosuccinate or sodium lauryl sulfate. METHOD 508.3 19 JUIY 1983 508.3-11