Downloaded from https://www.everyspec.com , MIL-STD-810D 19 JUIY 1983 ., (4? After the incubation period, check the fungal growth. NOTE: “The absence of oopious growth of any of the test organisma over the entire,surface in each container will invalidate the results of any teats using these spores. ., .. b~ ~mber environmmt .+, -, . + (1) Prepare the following solution: ; (a) 10.Og glycerol. ,. (b) 0.1g potassium dihydrogen orthophosphate (KH2P04). (c) O.Ig ammonium nitrate (NHUN03). (d) O .025g magnesium sulfate heptahydrate (MgS04‘7H20). (e) 0.05g yeast extract. (f) Distilled water to a total volume of 100 ml.
(g) O .005s of a nontoxic wetting agent such as sodium dioctyl &lfosuccinate or sodium lauryl sulfate. (h) HC1’and NaOH to adjust the final solution pH to 5.3. (2) Dip cottoristrips conforming to MIL-T-43566A (Tape, Textile, Cotton, General Purpose, Natural or .iflColors, Type la, Class 2, bleached, white flat construction) into the above solution. After dipping, remove the excess liquid from the strips and hang them to ,drybefore placing them in the chamber and inoculating. (3) Within the chamber, place the strips vertically close to and bracketing the test items so that the test strips and test items experience the same test environment.
The ,length,of the .stripashall be at least the height of the test item. ,,. (4) These strips,are installed and’inoculated along with”the test item to insure that proper conditions are present in the incubation chamber promote fungal growth. to II-3.2 ~ ,, 11-3.2.1 . . a. Assure that the condition of the items subjected to testing is ,similar to their condition as delivered by the manufacturer or customer for use, or as otherwise specified. Any cleaning of the test item shall be accomplished at -., least ‘f2hours before the beginning of the fungus test. b. Install the test item in the chamber or cabinet on suitable fixtures or suspended from hangers. hETHOD 508.3 508.3-13 19 JUIY 1983