INTRODUCTION 1.4 Vaccine Handling and Storage For All Vaccines: » Vaccines should arrive with cool packs in a well-insulated box. Figure 1. Varying presentations of inactivated vaccines and which are safe to use. » If vaccines arrive hot, call manufacturer or distributor. » Storage temperatures should be 2 to 7 єC (35 to 45 єF). » Avoid extreme heating and intense light. For Live Vaccines: » Transport to farm in coolers with ice packs to keep temperature constant. COBB VACCINATION MANAGEMENT GUIDE » Mix with diluent (reconstitute) just before application. » Use vaccine within 45 minutes after dilution for Marek’s vaccine and up to 2 hours for Infectious Bursal Disease and Newcastle Disease.
For Inactivated Vaccines: » Always follow the manufacturer’s instructions regarding preparation and delivery of any vaccine. » Inactivated vaccines are especially susceptible to temperature extremes or poor handling. These products are typically in an oil emulsion and mishandling these products can result in disruption of the emulsion, known as a broken emulsion. » Pre-warm oil emulsion vaccines at room temperature for 12 to 24 hours or using a warm water bath (do not exceed 32.2 єC (90 єF) for more than 5 hours). Pre-warming the vaccine reduces the viscosity of the mineral oil, making the administration easier and reducing any intense local reactions.
» Gently agitate bottles thoroughly prior to use. If the vaccine still has separate layers after agitating, test to see if the emulsion is broken, by shaking the bottle vigorously for 2 minutes. Let the bottle rest for 15 to 20 minutes. If separation persists, do not use that bottle of the vaccine and contact the manufacturer (See Figure 1). » Do not leave bottles in direct sunlight during transport to farm. 5 1 2 3 4 5 1. Normal 2. Normal 3. Normal 4. Broken Emulsion 5.
Broken Emulsion Uniform color; milky white Signficant settling has occurred Slight settling has occurred Bottom layer is dark brown or black Bottom layer 1 layer 2 layers 2 layers 3 layers 3 layers Ok to use Ok to use Ok to use Do not use! Do not use!