HATCHERY VACCINATION 2.2 Marek’s Vaccine Preparation Use only a clean and sanitized room for the preparation and reconstitution of the vaccine. Preferably, the vaccine should be prepared in a room located away from chick rooms to prevent possible chick dust contamination and limit any unnecessary people entering or exiting the area while vaccine is being prepared. Only designated and trained personnel should perform vaccine reconstitution. Before and after every batch of vaccine is reconstituted, the work area should be cleaned and sanitized. 1. All additives (dye, antibiotics, etc.) must be added to the diluent at least 5 minutes prior to adding vaccine.
For each additive, use a sterile syringe. Add vaccine dye to the diluent, then add antibiotic (only when prescribed by a licensed veterinarian and at the recommended dose). 2. Record all additives on the diluent bag. COBB VACCINATION DIGITAL MANAGEMENT GUIDE 3. Prepare a clean water bath with a chlorine disinfectant (final concentration of 200 ppm). Set water temperature to 27 єC (80 єF). 4. Remove the vials to be reconstituted from the liquid nitrogen tank. 5. Place the vials in the prepared water bath 27єC (80єF) and allow to thaw (approximately 70 to 90 seconds depending on the dose). 6. Once thawed, remove the vials from the water bath and dry using a clean paper towel. 7.
Spray or wipe the vials with 70% alcohol. Then, break the cap off the vial, taking care not to touch the vial openings (top and bottom). 8. Wipe the port of the diluent bag with a 70% alcohol wipe before withdrawal of the diluent. Using a sterile 20 ml syringe with 18-gauge needle, draw approximately 10 ml of prepared diluent (containing additives) from the diluent bag. This will act as a buffer for the vaccine. 9. Gently tap the top of the vial to ensure all the vaccine is in the bottom of the vial. Use an ampule opener or clean paper towel to avoid injury when opening the ampule.
Using the pre-prepared syringe containing 10 ml of the diluent and additives, gently draw the vaccine from all the vials (approximately 3 seconds per vial), and gently insert into the diluent bag (approximately 3 seconds per vial used). Take care not to withdraw or expel the vaccine too quickly with the syringe as this can cause damage to the vaccine due to excessive force on the cells which can reduce the potency of the vaccine. Agitate by gently tapping the top of the bag while injecting the vaccine into the bag. 7